RAC1 antibody

Catalog No. ABIN7118249

The Mouse Monoclonal anti-RAC1 antibody is suitable to detect RAC1 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IHC, IF and IP.

Quick Overview for RAC1 antibody (ABIN7118249)

Target: RAC1

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This RAC1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: 5D9

Product Details anti-RAC1 Antibody

Purpose: RAC1 antibody

Purification: Protein A+G purification

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: ras-related C3 botulinum toxin substrate 1(rho family, small GTP binding protein Rac1)

Isotype: IgG1

Application Details

Application Notes: WB: 1:1000-1:4000, IP: 1:200-1:2000, IHC: 1:50-1:500, IF: 1:50-1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for RAC1

Target: RAC1

Background:

Synonyms: Ras-related C3 botulinum toxin substrate 1|Cell migration-inducing gene 5 protein|Ras-like protein TC25|p21-Rac1|RAC1|TC25

Background: RAC1b, also named as TC25, belongs to the small GTPase superfamily and Rho family. RAC1 is a plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, it binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles.

Molecular Weight: 21 kDa

Gene ID: 5879

UniProt: P63000

Pathways: WNT Signaling, Regulation of Actin Filament Polymerization, Cell-Cell Junction Organization, Thromboxane A2 Receptor Signaling, VEGF Signaling