TRIM27 antibody (AA 200-513)

Catalog No. ABIN7118412

This anti-TRIM27 antibody is a Rabbit Polyclonal antibody detecting TRIM27 in WB, IHC, ELISA, IF and IP. Suitable for Human, Mouse and Rat.

Quick Overview for TRIM27 antibody (AA 200-513) (ABIN7118412)

Target: TRIM27 (Tripartite Motif Containing 27 (TRIM27))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRIM27 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP)

Product Details anti-TRIM27 Antibody

Binding Specificity: AA 200-513

Purpose: TRIM27 antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen:

Immunogen sequence: 200-513aa

Immunogen: tripartite motif-containing 27

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:2000, IP: 1:500-1:5000, IHC: 1:20-1:200, IF: 1:20-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for TRIM27

Target: TRIM27 (Tripartite Motif Containing 27 (TRIM27))

Alternative Name: TRIM27

Background:

Synonyms: Zinc finger protein RFP|RING finger protein 76|Ret finger protein|Tripartite motif-containing protein 27|TRIM27|RFP|RNF76

Background: E3 ubiquitin-protein ligase that mediates ubiquitination of PIK3C2B and inhibits its activity, mediates the formation of 'Lys-48'-linked polyubiquitin chains, the function inhibits CD4 T-cell activation. Acts as a regulator of retrograde transport: together with MAGEL2, mediates the formation of 'Lys-63'-linked polyubiquitin chains at 'Lys-220' of WASH1, leading to promote endosomal F-actin assembly(PubMed:23452853). Has a transcriptional repressor activity by cooperating with EPC1. Induces apoptosis by activating Jun N-terminal kinase and p38 kinase and also increases caspase-3-like activity independently of mitochondrial events. May function in male germ cell development. Has DNA-binding activity and preferentially bound to double-stranded DNA.

Molecular Weight: 58 kDa

Gene ID: 5987

UniProt: P14373