RPE65 antibody

Catalog No. ABIN7118420

The Rabbit Polyclonal anti-RPE65 antibody has been validated for WB and ELISA. It is suitable to detect RPE65 in samples from Human, Rat and Mouse.

Quick Overview for RPE65 antibody (ABIN7118420)

Target: RPE65 (Retinal Pigment Epithelium-Specific Protein 65kDa (RPE65))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RPE65 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-RPE65 Antibody

Purpose: RPE65 antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: retinal pigment epithelium-specific protein 65kDa

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:5000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for RPE65

Target: RPE65 (Retinal Pigment Epithelium-Specific Protein 65kDa (RPE65))

Alternative Name: RPE65

Background:

Synonyms: Retinoid isomerohydrolase|All-trans-retinyl-palmitate hydrolase|Lutein isomerase|Meso-zeaxanthin isomerase|Retinal pigment epithelium-specific 65 kDa protein|Retinol isomerase|RPE65

Background: Plays important roles in the production of 11-cis retinal and in visual pigment regeneration. The soluble form binds vitamin A(all-trans-retinol), making it available for LRAT processing to all-trans-retinyl ester. The membrane form, palmitoylated by LRAT, binds all-trans-retinyl esters, making them available for IMH(isomerohydrolase) processing to all-cis-retinol. The soluble form is regenerated by transferring its palmitoyl groups onto 11-cis-retinol, a reaction catalyzed by LRAT. The enzymatic activity is linearly dependent of the expression levels and membrane association.

Molecular Weight: 65 kDa, 61 kDa

Gene ID: 6121

UniProt: Q16518