RFFL antibody

Catalog No. ABIN7118629

This Rabbit Polyclonal antibody specifically detects RFFL in WB and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for RFFL antibody (ABIN7118629)

Target: RFFL (Ring Finger and FYVE-Like Domain Containing 1 (RFFL))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RFFL antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-RFFL Antibody

Purpose: RFFL antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: ring finger and FYVE-like domain containing 1

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:2000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for RFFL

Target: RFFL (Ring Finger and FYVE-Like Domain Containing 1 (RFFL))

Alternative Name: RFFL

Background:

Synonyms: E3 ubiquitin-protein ligase rififylin|Caspase regulator CARP2|Caspases-8 and -10-associated RING finger protein 2 (CARP-2)|FYVE-RING finger protein Sakura (Fring)|RING finger and FYVE-like domain-containing protein 1|RING finger protein 189|RING finger protein 34-like|RING-type E3 ubiquitin transferase rififylin|RFFL|RNF189|RNF34L

Background: E3 ubiquitin-protein ligase that regulates several biological processes through the ubiquitin-mediated proteasomal degradation of various target proteins. Mediates 'Lys-48'-linked polyubiquitination of PRR5L and its subsequent proteasomal degradation thereby indirectly regulating cell migration through the mTORC2 complex. Ubiquitinates the caspases CASP8 and CASP10, promoting their proteasomal degradation, to negatively regulate cell death downstream of death domain receptors in the extrinsic pathway of apoptosis. Negatively regulates the tumor necrosis factor-mediated signaling pathway through targeting of RIPK1 to ubiquitin-mediated proteasomal degradation. Negatively regulates p53/TP53 through its direct ubiquitination and targeting to proteasomal degradation. Indirectly, may also negatively regulate p53/TP53 through ubiquitination and degradation of SFN. May also play a role in endocytic recycling.

Molecular Weight: 37-41 kDa

Gene ID: 117584

UniProt: Q8WZ73