RLIM antibody

Catalog No. ABIN7118663

The Rabbit Polyclonal anti-RLIM antibody is suitable to detect RLIM in samples from Human, Mouse and Rat. It has been validated for WB, ELISA and IP.

Quick Overview for RLIM antibody (ABIN7118663)

Target: RLIM (Ring Finger Protein, LIM Domain Interacting (RLIM))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RLIM antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Product Details anti-RLIM Antibody

Purpose: RLIM antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: ring finger protein, LIM domain interacting

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:2000, IP: 1:200-1:2000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for RLIM

Target: RLIM (Ring Finger Protein, LIM Domain Interacting (RLIM))

Alternative Name: RLIM

Background:

Synonyms: E3 ubiquitin-protein ligase RLIM|LIM domain-interacting RING finger protein|RING finger LIM domain-binding protein (R-LIM)|RING finger protein 12|RING-type E3 ubiquitin transferase RLIM|Renal carcinoma antigen NY-REN-43|RLIM|RNF12

Background: E3 ubiquitin-protein ligase. Acts as a negative coregulator for LIM homeodomain transcription factors by mediating the ubiquitination and subsequent degradation of LIM cofactors LDB1 and LDB2 and by mediating the recruitment the SIN3a/histone deacetylase corepressor complex. Ubiquitination and degradation of LIM cofactors LDB1 and LDB2 allows DNA-bound LIM homeodomain transcription factors to interact with other protein partners such as RLIM. Plays a role in telomere length-mediated growth suppression by mediating the ubiquitination and degradation of TERF1. By targeting ZFP42 for degradation, acts as an activator of random inactivation of X chromosome in the embryo, a stochastic process in which one X chromosome is inactivated to minimize sex-related dosage differences of X-encoded genes in somatic cells of female placental mammals.

Molecular Weight: 75 kDa

Gene ID: 51132

UniProt: Q9NVW2