RUNX1 antibody

Catalog No. ABIN7118733

This anti-RUNX1 antibody is a Rabbit Polyclonal antibody detecting RUNX1 in WB, ELISA and IP. Suitable for Human, Mouse and Rat.

Quick Overview for RUNX1 antibody (ABIN7118733)

Target: RUNX1 (Runt-Related Transcription Factor 1 (RUNX1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RUNX1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Product Details anti-RUNX1 Antibody

Purpose: RUNX1 antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: runt-related transcription factor 1

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:2000, IP: 1:200-1:2000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for RUNX1

Target: RUNX1 (Runt-Related Transcription Factor 1 (RUNX1))

Alternative Name: RUNX1

Background:

Synonyms: Runt-related transcription factor 1|Acute myeloid leukemia 1 protein|Core-binding factor subunit alpha-2 (CBF-alpha-2)|Oncogene AML-1|Polyomavirus enhancer-binding protein 2 alpha B subunit (PEA2-alpha B, PEBP2-alpha B)|SL3-3 enhancer factor 1 alpha B subunit|SL3/AKV core-binding factor alpha B subunit|RUNX1|AML1|CBFA2

Background: CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL-3 and GM-CSF promoters. The alpha subunit binds DNA and appears to have a role in the development of normal hematopoiesis. Isoform AML-1L interferes with the transactivation activity of RUNX1. Acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the mouse BLK promoter. Inhibits KAT6B-dependent transcriptional activation. Controls the anergy and suppressive function of regulatory T-cells(Treg) by associating with FOXP3. Activates the expression of IL2 and IFNG and down-regulates the expression of TNFRSF18, IL2RA and CTLA4, in conventional T-cells(PubMed:17377532).

Molecular Weight: 48-55 kDa

Gene ID: 861

UniProt: Q01196