SETMAR antibody

Catalog No. ABIN7118987

The Rabbit Polyclonal anti-SETMAR antibody is suitable to detect SETMAR in samples from Human. It has been validated for ELISA, WB and IP.

Quick Overview for SETMAR antibody (ABIN7118987)

Target: SETMAR (SET Domain and Mariner Transposase Fusion Gene (SETMAR))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SETMAR antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-SETMAR Antibody

Purpose: SETMAR antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: SET domain and mariner transposase fusion gene

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:2000, IP: 1:200-1:2000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for SETMAR

Target: SETMAR (SET Domain and Mariner Transposase Fusion Gene (SETMAR))

Alternative Name: SETMAR

Background:

Synonyms: Histone-lysine N-methyltransferase SETMAR|SET domain and mariner transposase fusion protein (Metnase)|SETMAR

Background: Protein derived from the fusion of a methylase with the transposase of an Hsmar1 transposon that plays a role in DNA double-strand break repair, stalled replication fork restart and DNA integration. DNA-binding protein, it is indirectly recruited to sites of DNA damage through protein-protein interactions. Has also kept a sequence-specific DNA-binding activity recognizing the 19-mer core of the 5'-terminal inverted repeats(TIRs) of the Hsmar1 element and displays a DNA nicking and end joining activity(PubMed:16332963, PubMed:16672366, PubMed:17877369, PubMed:17403897, PubMed:18263876, PubMed:22231448, PubMed:24573677, PubMed:20521842). In parallel, has a histone methyltransferase activity and methylates 'Lys-4' and 'Lys-36' of histone H3. Specifically mediates dimethylation of H3 'Lys-36' at sites of DNA double-strand break and may recruit proteins required for efficient DSB repair through non-homologous end-joining(PubMed:16332963, PubMed:21187428, PubMed:22231448). Also regulates replication fork processing, promoting replication fork restart and regulating DNA decatenation through stimulation of the topoisomerase activity of TOP2A(PubMed:18790802, PubMed:20457750).

Molecular Weight: 77 kDa

Gene ID: 6419

UniProt: Q53H47

Pathways: Positive Regulation of Response to DNA Damage Stimulus