SIX Homeobox 1 antibody

Catalog No. ABIN7119116

The Rabbit Polyclonal anti-SIX Homeobox 1 antibody has been validated for WB, ELISA, IF and IP. It is suitable to detect SIX Homeobox 1 in samples from Human and Mouse.

Quick Overview for SIX Homeobox 1 antibody (ABIN7119116)

Target: SIX Homeobox 1 (SIX1)

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SIX Homeobox 1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP)

Product Details anti-SIX Homeobox 1 Antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: SIX homeobox 1

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:2000, IP: 1:200-1:1000, IF: 1:20-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

Expiry Date: 12 months

Target Details for SIX Homeobox 1

Target: SIX Homeobox 1 (SIX1)

Alternative Name: SIX1

Background: Synonyms:BOS3, DFNA23, Homeobox protein SIX1, Sine oculis homeobox homolog 1, SIX homeobox 1, SIX1, TIP39 Background:Transcription factor that is involved in the regulation of cell proliferation, apoptosis and embryonic development. Plays an important role in the development of several organs, including kidney, muscle and inner ear. Depending on context, functions as transcriptional repressor or activator. Lacks an activation domain, and requires interaction with EYA family members for transcription activation. Mediates nuclear translocation of EYA1 and EYA2. Binds the 5'-TCA[AG][AG]TTNC-3' motif present in the MEF3 element in the MYOG promoter. Regulates the expression of numerous genes, including MYC, CCND1 and EZR. Acts as activator of the IGFBP5 promoter, probably coactivated by EYA2. Repression of precursor cell proliferation in myoblasts is switched to activation through recruitment of EYA3 to the SIX1-DACH1 complex. During myogenesis, seems to act together with EYA2 and DACH2(By similarity). Regulates the expression of CCNA1.

Molecular Weight: 32-35 kDa

Gene ID: 6495

UniProt: Q15475

Pathways: Sensory Perception of Sound, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development