SF3B2 antibody

Catalog No. ABIN7119435

The Rabbit Polyclonal anti-SF3B2 antibody (ABIN7119435) specifically detects SF3B2 in WB, IHC, ELISA and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for SF3B2 antibody (ABIN7119435)

Target: SF3B2 (Splicing Factor 3b, Subunit 2, 145kDa (SF3B2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SF3B2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-SF3B2 Antibody

Purpose: SF3B2 antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: splicing factor 3b, subunit 2, 145kDa

Isotype: IgG

Application Details

Application Notes: WB: 1:500 - 1:2000, IHC: 1:50 - 1:200, IF: 1:50 - 1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for SF3B2

Target: SF3B2 (Splicing Factor 3b, Subunit 2, 145kDa (SF3B2))

Alternative Name: SF3B2

Background:

Synonyms: Splicing factor 3B subunit 2|Pre-mRNA-splicing factor SF3b 145 kDa subunit (SF3b145)|Spliceosome-associated protein 145 (SAP 145)|SF3B2|SAP145

Background: This gene encodes subunit 2 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence-independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 2 associates with pre-mRNA upstream of the branch site at the anchoring site. Subunit 2 also interacts directly with subunit 4 of the splicing factor 3b complex. Subunit 2 is a highly hydrophilic protein with a proline-rich N-terminus and a glutamate-rich stretch in the C-terminus.

Molecular Weight: 140 kDa

Gene ID: 10992

UniProt: Q13435