TJP1 antibody (AA 480-742)

Catalog No. ABIN7119969

The Rabbit Polyclonal anti-TJP1 antibody (ABIN7119969) specifically detects TJP1 in WB, IHC, ELISA, IP, IF and FACS. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for TJP1 antibody (AA 480-742) (ABIN7119969)

Target: TJP1 (Tight Junction Protein 1 (TJP1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TJP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-TJP1 Antibody

Binding Specificity: AA 480-742

Purpose: TJP1 antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen:

Immunogen sequence: 480-742aa

Immunogen: tight junction protein 1(zona occludens 1)

Isotype: IgG

Application Details

Application Notes: WB: 1:200-1:2000, IP: 1:200-1:2000, IHC: 1:50-1:200, IF: 1:20-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for TJP1

Target: TJP1 (Tight Junction Protein 1 (TJP1))

Alternative Name: TJP1

Background:

Synonyms: Tight junction protein ZO-1|Tight junction protein 1|Zona occludens protein 1|Zonula occludens protein 1|TJP1|ZO1

Background: Tight junction(or zonula occludens) form the continuous intercellular barrier between epithelial and endothelial cells, which is required to separate tissue spaces and regulate selective movement of solutes across the epithelium and endothelium(PMID: 20066090). ZO-1(also known as TJP1) is a peripheral membrane phosphoprotein located on the cytoplasmic face and is expressed in tight junctions of both epithelial and endothelial cells(PMID: 3528172). It binds the transmembrane proteins occludin and the claudins linking them to cytoskeletal actin(PMID: 17418867). ZO-1 belongs to a family of multidomain proteins known as the membrane-associated guanylate kinase homologs(MAGUKs). It is a pivotal tight junction protein and may be involved in signalling mechanisms regulating cell proliferation and differentiation(PMID: 22782886).

Molecular Weight: 230 kDa

Gene ID: 7082

UniProt: Q07157

Pathways: Carbohydrate Homeostasis, Cell-Cell Junction Organization