TPX2 antibody (AA 1-256)

Catalog No. ABIN7120029

This anti-TPX2 antibody is a Rabbit Polyclonal antibody detecting TPX2 in WB, ELISA, IP and IF. Suitable for Human, Mouse and Rat.

Quick Overview for TPX2 antibody (AA 1-256) (ABIN7120029)

Target: TPX2 (TPX2, Microtubule-Associated, Homolog (Xenopus Laevis) (TPX2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TPX2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF)

Product Details anti-TPX2 Antibody

Binding Specificity: AA 1-256

Purpose: TPX2 antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen:

Immunogen sequence: 1-256aa

Immunogen: TPX2, microtubule-associated, homolog(Xenopus laevis)

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:2000, IP: 1:200-1:1000, IF: 1:20-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for TPX2

Target: TPX2 (TPX2, Microtubule-Associated, Homolog (Xenopus Laevis) (TPX2))

Alternative Name: TPX2

Background:

Synonyms: Targeting protein for Xklp2|Differentially expressed in cancerous and non-cancerous lung cells 2 (DIL-2)|Hepatocellular carcinoma-associated antigen 519|Hepatocellular carcinoma-associated antigen 90|Protein fls353|Restricted expression proliferation-associated protein 100 (p100)|TPX2|C20orf1|C20orf2|DIL2|HCA519

Background: Spindle assembly factor required for normal assembly of mitotic spindles. Required for normal assembly of microtubules during apoptosis. Required for chromatin and/or kinetochore dependent microtubule nucleation. Mediates AURKA localization to spindle microtubules(PubMed:18663142, PubMed:19208764). Activates AURKA by promoting its autophosphorylation at 'Thr-288' and protects this residue against dephosphorylation(PubMed:18663142, PubMed:19208764). TPX2 is inactivated upon binding to importin-alpha(PubMed:26165940). At the onset of mitosis, GOLGA2 interacts with importin-alpha, liberating TPX2 from importin-alpha, allowing TPX2 to activates AURKA kinase and stimulates local microtubule nucleation(PubMed:26165940).

Molecular Weight: 100 kDa

Gene ID: 22974

UniProt: Q9ULW0