TRIM5 antibody

Catalog No. ABIN7120293

The Rabbit Polyclonal anti-TRIM5 antibody (ABIN7120293) specifically detects TRIM5 in WB, ELISA and IHC. The antibody is reactive with Human and Mouse samples.

Quick Overview for TRIM5 antibody (ABIN7120293)

Target: TRIM5 (Tripartite Motif Containing 5 (TRIM5))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRIM5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-TRIM5 Antibody

Purpose: TRIM5 antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: tripartite motif-containing 5

Isotype: IgG

Application Details

Application Notes: WBL:1:200-1:1000, IHC: 1:20-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for TRIM5

Target: TRIM5 (Tripartite Motif Containing 5 (TRIM5))

Alternative Name: TRIM5

Background:

Synonyms: Tripartite motif-containing protein 5|RING finger protein 88|RING-type E3 ubiquitin transferase TRIM5|TRIM5|RNF88

Background: Capsid-specific restriction factor that prevents infection from non-host-adapted retroviruses. Blocks viral replication early in the life cycle, after viral entry but before reverse transcription. In addition to acting as a capsid-specific restriction factor, also acts as a pattern recognition receptor that activates innate immune signaling in response to the retroviral capsid lattice. Binding to the viral capsid triggers its E3 ubiquitin ligase activity, and in concert with the heterodimeric ubiquitin conjugating enzyme complex UBE2V1-UBE2N(also known as UBC13-UEV1A complex) generates 'Lys-63'-linked polyubiquitin chains, which in turn are catalysts in the autophosphorylation of the MAP3K7/TAK1 complex(includes TAK1, TAB2, and TAB3). Activation of the MAP3K7/TAK1 complex by autophosphorylation results in the induction and expression of NF-kappa-B and MAPK-responsive inflammatory genes, thereby leading to an innate immune response in the infected cell. Restricts infection by N-tropic murine leukemia virus(N-MLV), equine infectious anemia virus(EIAV), simian immunodeficiency virus of macaques(SIVmac), feline immunodeficiency virus(FIV), and bovine immunodeficiency virus(BIV)(PubMed:17156811). Plays a role in regulating autophagy through activation of autophagy regulator BECN1 by causing its dissociation from its inhibitors BCL2 and TAB2(PubMed:25127057). Also plays a role in autophagy by acting as a selective autophagy receptor which recognizes and targets HIV-1 capsid protein p24 for autophagic destruction(PubMed:25127057).

Molecular Weight: 70 kDa, 46 kDa

Gene ID: 85363

UniProt: Q9C035

Pathways: Activation of Innate immune Response