USP9X antibody

Catalog No. ABIN7120533

The Rabbit Polyclonal anti-USP9X antibody (ABIN7120533) specifically detects USP9X in WB, ELISA, IHC, IF and IP. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for USP9X antibody (ABIN7120533)

Target: USP9X (Ubiquitin Specific Peptidase 9, X-Linked (USP9X))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This USP9X antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Product Details anti-USP9X Antibody

Purpose: USP9X antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: ubiquitin specific peptidase 9, X-linked

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:5000, IF: 1:10-1:100, IHC: 1:20-1:200, IP: 1:200-1:2000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for USP9X

Target: USP9X (Ubiquitin Specific Peptidase 9, X-Linked (USP9X))

Alternative Name: USP9X

Background:

Synonyms: Probable ubiquitin carboxyl-terminal hydrolase FAF-X|Deubiquitinating enzyme FAF-X|Fat facets in mammals (hFAM)|Fat facets protein-related, X-linked|Ubiquitin thioesterase FAF-X|Ubiquitin-specific protease 9, X chromosome|Ubiquitin-specific-processing protease FAF-X|USP9X|DFFRX|FAM|USP9

Background: Deubiquitinase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. May therefore play an important regulatory role at the level of protein turnover by preventing degradation of proteins through the removal of conjugated ubiquitin. Essential component of TGF-beta/BMP signaling cascade. Regulates chromosome alignment and segregation in mitosis by regulating the localization of BIRC5/survivin to mitotic centromeres. Specifically hydrolyzes both 'Lys-29'-and 'Lys-33'-linked polyubiquitins chains. Specifically deubiquitinates monoubiquitinated SMAD4, opposing the activity of E3 ubiquitin-protein ligase TRIM33. Involved in axonal growth and neuronal cell migration.

Molecular Weight: 260-290 kDa

Gene ID: 8239

UniProt: Q93008