UBE2K antibody

Catalog No. ABIN7120554

The Rabbit Polyclonal anti-UBE2K antibody (ABIN7120554) specifically detects UBE2K in WB, ELISA and IHC. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for UBE2K antibody (ABIN7120554)

Target: UBE2K (Ubiquitin-Conjugating Enzyme E2K (UBE2K))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBE2K antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-UBE2K Antibody

Purpose: UBE2K antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: ubiquitin-conjugating enzyme E2K

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:2000, IHC: 1:20-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for UBE2K

Target: UBE2K (Ubiquitin-Conjugating Enzyme E2K (UBE2K))

Alternative Name: UBE2K

Background:

Synonyms: Ubiquitin-conjugating enzyme E2 K|E2 ubiquitin-conjugating enzyme K|Huntingtin-interacting protein 2 (HIP-2)|Ubiquitin carrier protein|Ubiquitin-conjugating enzyme E2-25 kDa (Ubiquitin-conjugating enzyme E2(25K), Ubiquitin-conjugating enzyme E2-25K)|Ubiquitin-protein ligase|UBE2K|HIP2|LIG

Background: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro, in the presence or in the absence of BRCA1-BARD1 E3 ubiquitin-protein ligase complex, catalyzes the synthesis of 'Lys-48'-linked polyubiquitin chains. Does not transfer ubiquitin directly to but elongates monoubiquitinated substrate protein. Mediates the selective degradation of short-lived and abnormal proteins, such as the endoplasmic reticulum-associated degradation(ERAD) of misfolded lumenal proteins. Ubiquitinates huntingtin. May mediate foam cell formation by the suppression of apoptosis of lipid-bearing macrophages through ubiquitination and subsequence degradation of p53/TP53. Proposed to be involved in ubiquitination and proteolytic processing of NF-kappa-B, in vitro supports ubiquitination of NFKB1. In case of infection by cytomegaloviruses may be involved in the US11-dependent degradation of MHC class I heavy chains following their export from the ER to the cytosol. In case of viral infections may be involved in the HPV E7 protein-dependent degradation of RB1.

Molecular Weight: 25 kDa

Gene ID: 3093

UniProt: P61086