VPS35 antibody

Catalog No. ABIN7120708

Product Details anti-VPS35 Antibody

Target: VPS35 (Vacuolar Protein Sorting 35 (VPS35))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VPS35 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), ELISA

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: vacuolar protein sorting 35 homolog(S. cerevisiae)

Isotype: IgG

Application Details

Application Notes: WB: 1:200-1:1000, IP: 1:200-1:1000, IF: 1:10-1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

Expiry Date: 12 months

Target Details for VPS35

Target: VPS35 (Vacuolar Protein Sorting 35 (VPS35))

Alternative Name: VPS35 (VPS35 Products)

Synonyms: MEM3 antibody, PARK17 antibody, vps35 antibody, MGC81959 antibody, bZ34G2.3 antibody, si:rp71-34g2.3 antibody, wu:fa66e03 antibody, wu:fb95c08 antibody, zgc:136268 antibody, 23.m05999 antibody, Mem3 antibody, VPS35 antibody, DKFZp459E1510 antibody, CG5625 antibody, DVps35 antibody, Dmel\\CG5625 antibody, Dvps35 antibody, dvps35 antibody, AI647796 antibody, mem3 antibody, VPS35, retromer complex component antibody, VPS35 retromer complex component L homeolog antibody, VPS35 retromer complex component antibody, vacuolar protein sorting 35 homolog (S. cerevisiae) antibody, vacuolar protein sorting-associated protein 35 antibody, vacuolar protein sorting 35 antibody, Vacuolar protein sorting 35 antibody, hypothetical protein antibody, VPS35 retromer complex component S homeolog antibody, retromer complex subunit Vps35 antibody, VPS35 antibody, vps35.L antibody, vps35 antibody, Tc00.1047053511367.170 antibody, Tb10.70.5460 antibody, BBOV_IV009740 antibody, CC1G_12185 antibody, PTRG_08682 antibody, CMU_017400 antibody, PHATR_43830 antibody, MCYG_00751 antibody, MGYG_01212 antibody, EHI_086580 antibody, EHI_002990 antibody, EHI_041950 antibody, EHI_096520 antibody, Bm1_44045 antibody, LOC100285267 antibody, Vps35 antibody, PGTG_10018 antibody, Tsp_09825 antibody, vps35.S antibody

Background: Synonyms:DKFZp434E1211, DKFZp434P1672, FLJ10752, FLJ13588, FLJ20388, hVPS35, Maternal embryonic 3, MEM3, Vesicle protein sorting 35, VPS35 Background:Acts as component of the retromer cargo-selective complex(CSC). The CSC is believed to be the core functional component of retromer or respective retromer complex variants acting to prevent missorting of selected transmembrane cargo proteins into the lysosomal degradation pathway. The recruitment of the CSC to the endosomal membrane involves RAB7A and SNX3. The CSC seems to associate with the cytoplasmic domain of cargo proteins predominantly via VPS35, however, these interactions seem to be of low affinity and retromer SNX proteins may also contribute to cargo selectivity thus questioning the classical function of the CSC. The SNX-BAR retromer mediates retrograde transport of cargo proteins from endosomes to the trans-Golgi network(TGN) and is involved in endosome-to-plasma membrane transport for cargo protein recycling. The SNX3-retromer mediates the retrograde endosome-to-TGN transport of WLS distinct from the SNX-BAR retromer pathway. The SNX27-retromer is believed to be involved in endosome-to-plasma membrane trafficking and recycling of a broad spectrum of cargo proteins. The CSC seems to act as recruitment hub for other proteins, such as the WASH complex and TBC1D5(Probable). Required for retrograde transport of lysosomal enzyme receptor IGF2R and SLC11A2. Required to regulate transcytosis of the polymeric immunoglobulin receptor(pIgR-pIgA)(PubMed:15078903, PubMed:15247922, PubMed:20164305). Required for endosomal localization of FAM21C(PubMed:22070227). Mediates the association of the CSC with the WASH complex via FAM21(PubMed:22070227, PubMed:24980502, PubMed:24819384). Required for the endosomal localization of TBC1D5(PubMed:20923837).

Molecular Weight: 92 kDa

Gene ID: 55737

UniProt: Q96QK1