VPS16 antibody

Catalog No. ABIN7120720

This Rabbit Polyclonal antibody specifically detects VPS16 in WB, ELISA, IHC and IP. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for VPS16 antibody (ABIN7120720)

Target: VPS16 (Vacuolar Protein Sorting-Associated Protein 16 Homolog (VPS16))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VPS16 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)

Product Details anti-VPS16 Antibody

Purpose: VPS16 antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: vacuolar protein sorting 16 homolog

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:5000, IHC: 1:20-1:200, IP: 1:200-1:2000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for VPS16

Target: VPS16 (Vacuolar Protein Sorting-Associated Protein 16 Homolog (VPS16))

Alternative Name: VPS16

Background:

Synonyms: Vacuolar protein sorting-associated protein 16 homolog (hVPS16)|VPS16

Background: Plays a role in vesicle-mediated protein trafficking to lysosomal compartments including the endocytic membrane transport and autophagic pathways. Believed to act as a core component of the putative HOPS and CORVET endosomal tethering complexes which are proposed to be involved in the Rab5-to-Rab7 endosome conversion probably implicating MON1A/B, and via binding SNAREs and SNARE complexes to mediate tethering and docking events during SNARE-mediated membrane fusion. The HOPS complex is proposed to be recruited to Rab7 on the late endosomal membrane and to regulate late endocytic, phagocytic and autophagic traffic towards lysosomes. The CORVET complex is proposed to function as a Rab5 effector to mediate early endosome fusion probably in specific endosome subpopulations(PubMed:11382755, PubMed:23351085, PubMed:24554770, PubMed:25266290, PubMed:25783203). Required for recruitment of VPS33A to the HOPS complex(PubMed:23901104). Required for fusion of endosomes and autophagosomes with lysosomes, the function is dependent on its association with VPS33A but not VPS33B(PubMed:25783203). The function in autophagosome-lysosome fusion implicates STX17 but not UVRAG(PubMed:24554770).

Molecular Weight: 95 kDa

Gene ID: 64601

UniProt: Q9H269