VEGFA antibody

Catalog No. ABIN7120740

This anti-VEGFA antibody is a Mouse Monoclonal antibody detecting VEGFA in WB, IHC and ELISA. Suitable for Human.

Quick Overview for VEGFA antibody (ABIN7120740)

Target: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This VEGFA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Clone: 5H5

Product Details anti-VEGFA Antibody

Purpose: VEGFA antibody

Purification: protein A+G purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: vascular endothelial growth factor A

Isotype: IgG2b

Application Details

Application Notes: WB: 1:1000-1:4000, IHC: 1:50-1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for VEGFA

Target: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Alternative Name: VEGFA

Background:

Synonyms: Vascular endothelial growth factor A, long form (L-VEGF)|Vascular permeability factor (VPF)|N-VEGF|VEGFA|VEGFA|VEGF

Background: VEGFA, also named as VEGF or VPF, belongs to the PDGF/VEGF growth factor family. It is a growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. VEGFA induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. It binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Defects in VEGFA are a cause of susceptibility to microvascular complications of diabetes type 1(MVCD1). VEGFA has 13 isoforms with MW 16kd to 45kd. Some isoforms has homodimer form, such as VEGFA189(38kd), VEFGA110(34kd). VEGFA is a 34kd to 42kd, dimeric, disulfide-bound glycoprotein. VEGF-A exists in at least seven homodimeric isoforms. The monomers consist of 121, 145, 148, 165, 183, 189, or 206 amino acids(PMID:15602010). This antibody can recognize all the isoforms of VEFGA.

Molecular Weight: 45 kDa

UniProt: P15692

Pathways: RTK Signaling, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling