Vinculin antibody

Catalog No. ABIN7120772

The Mouse Monoclonal anti-Vinculin antibody is suitable to detect Vinculin in samples from Human, Mouse and Rat. It has been validated for WB, IHC, ELISA, IF and FACS.

Quick Overview for Vinculin antibody (ABIN7120772)

Target: Vinculin (VCL)

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Vinculin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: 5E7

Product Details anti-Vinculin Antibody

Purpose: VCL antibody

Purification: Protein A+G purification

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: vinculin

Isotype: IgG1

Application Details

Application Notes: WB: 1:2000-1:20000, IHC: 1:50-1:500, IF: 1:50-1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for Vinculin

Target: Vinculin (VCL)

Alternative Name: VCL

Background:

Synonyms: Vinculin|Metavinculin (MV)|VCL

Background: Vinculin belongs to the vinculin/alpha-catenin family. It is an actin filament(F-actin)-binding protein which involved in cell-matrix adhesion and cell-cell adhesion. Vinculin regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. It may also play important roles in cell morphology and locomotion. Vinculin is a 117- kDa, 1,066-amino-acid protein which is ubiquitously expressed. Its splice variant, metavinculin(124 kDa), is muscle-specific.

Molecular Weight: 124 kDa

UniProt: P18206

Pathways: Cell-Cell Junction Organization, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2