GPR177/WLS antibody (AA 43-234)

Catalog No. ABIN7120857

This anti-GPR177/WLS antibody is a Rabbit Polyclonal antibody detecting GPR177/WLS in ELISA, WB, IHC and IP. Suitable for Human, Mouse and Rat.

Quick Overview for GPR177/WLS antibody (AA 43-234) (ABIN7120857)

Target: GPR177/WLS (WLS) (Wntless Homolog (WLS))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPR177/WLS antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Product Details anti-GPR177/WLS Antibody

Binding Specificity: AA 43-234

Purpose: WLS antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen:

Immunogen sequence: 43-234aa

Immunogen: G protein-coupled receptor 177

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:1000, IP: 1:500-1:1000, IHC: 1:20-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for GPR177/WLS

Target: GPR177/WLS (WLS) (Wntless Homolog (WLS))

Alternative Name: WLS

Background:

Synonyms: Protein wntless homolog|Integral membrane protein GPR177|Protein evenness interrupted homolog (EVI)|Putative NF-kappa-B-activating protein 373|WLS|C1orf139|GPR177

Background: GPR177, also known as Wntless (WLS) or Evi, is an evolutionarily conserved transmembrane protein necessary for Wnt protein secretion. GPR177 localizes to compartments of the secretory pathway, including the ER, Golgi apparatus, endosomes, and plasma membrane. GPR177 is overexpressed in several cancer types including glioma and gastric cancer. Several isoforms of GPR177 exist due to the alternative splicing, with predicted MW around 62 and 51 kDa, respectively. The sequence can be processed by removing the signal peptide, which generates a mature form of 45-46 kDa. In addition, slow migragrating band above 62 kDa can also be detected because GPR177 is modified by N-linked glycosylation (PMID: 20549736).

Molecular Weight: 46-62 kDa

Gene ID: 79971

UniProt: Q5T9L3

Pathways: WNT Signaling