YTHDF1 antibody (AA 133-349)

Catalog No. ABIN7120903

This Rabbit Polyclonal antibody specifically detects YTHDF1 in WB, ELISA, IHC and IP. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for YTHDF1 antibody (AA 133-349) (ABIN7120903)

Target: YTHDF1 (YTH Domain Family, Member 1 (YTHDF1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This YTHDF1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)

Product Details anti-YTHDF1 Antibody

Binding Specificity: AA 133-349

Purpose: YTHDF1 antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen:

Immunogen sequence: 133-349aa

Immunogen: YTH domain family, member 1

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:5000, IP: 1:500-1:5000, IHC: 1:20-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for YTHDF1

Target: YTHDF1 (YTH Domain Family, Member 1 (YTHDF1))

Alternative Name: YTHDF1

Background:

Synonyms: YTH domain-containing family protein 1 (DF1)|Dermatomyositis associated with cancer putative autoantigen 1 (DACA-1)|YTHDF1|C20orf21

Background: Specifically recognizes and binds N6-methyladenosine(m6A)-containing mRNAs, and promotes mRNA translation efficiency(PubMed:24284625, PubMed:26046440, PubMed:26318451). M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability(PubMed:24284625). Acts as a regulator of mRNA translation efficiency: promotes ribosome loading to m6A-containing mRNAs and interacts with translation initiation factors eIF3(EIF3A or EIF3B) to facilitate translation initiation(PubMed:26046440).

Molecular Weight: 62 kDa

Gene ID: 54915

UniProt: Q9BYJ9