YTHDF2 antibody

Catalog No. ABIN7120904

The Rabbit Polyclonal anti-YTHDF2 antibody has been validated for WB, ELISA, IHC, IF and IP. It is suitable to detect YTHDF2 in samples from Human, Mouse and Rat.

Quick Overview for YTHDF2 antibody (ABIN7120904)

Target: YTHDF2 (YTH Domain Family, Member 2 (YTHDF2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This YTHDF2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Product Details anti-YTHDF2 Antibody

Purpose: YTHDF2 antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: YTH domain family, member 2

Isotype: IgG

Application Details

Application Notes: WB: 1:1000-1:10000, IF: 1:10-1:100, IP: 1:200-1:1000, IHC: 1:20-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for YTHDF2

Target: YTHDF2 (YTH Domain Family, Member 2 (YTHDF2))

Alternative Name: YTHDF2

Background:

Synonyms: YTH domain-containing family protein 2 (DF2)|CLL-associated antigen KW-14|High-glucose-regulated protein 8|Renal carcinoma antigen NY-REN-2|YTHDF2|HGRG8

Background: Specifically recognizes and binds N6-methyladenosine(m6A)-containing RNAs, and regulates mRNA stability(PubMed:24284625, PubMed:26046440, PubMed:26318451). M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability(PubMed:22575960, PubMed:24284625, PubMed:25412658, PubMed:25412661). Acts as a regulator of mRNA stability: binding to m6A-containing mRNAs results in the localization to mRNA decay sites, such as processing bodies(P-bodies), leading to mRNA degradation(PubMed:24284625, PubMed:26046440). Also acts as a promoter of cap-independent mRNA translation following heat shock stress: upon stress, relocalizes to the nucleus and specifically binds mRNAs with some m6A methylation mark at their 5'-UTR, protecting demethylation of mRNAs by FTO, thereby promoting cap-independent mRNA translation(PubMed:26458103).

Molecular Weight: 68 kDa

Gene ID: 51441

UniProt: Q9Y5A9