PFKFB2 antibody (AA 51-150)

Catalog No. ABIN708251

The Rabbit Polyclonal anti-PFKFB2 antibody is suitable to detect PFKFB2 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IHC (p), IF (cc), IF (p) and IHC (fro).

Quick Overview for PFKFB2 antibody (AA 51-150) (ABIN708251)

Target: PFKFB2 (6-phosphofructo-2-Kinase/fructose-2,6-Biphosphatase 2 (PFKFB2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PFKFB2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-PFKFB2 Antibody

Binding Specificity: AA 51-150

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Dog,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human PFKFB2

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for PFKFB2

Target: PFKFB2 (6-phosphofructo-2-Kinase/fructose-2,6-Biphosphatase 2 (PFKFB2))

Alternative Name: PFKFB2

Background:

Synonyms: 6 phosphofructo 2 kinase/fructose 2 6 biphosphatase 2, 6-bisphosphatase, 6-P2ase 2, 6-P2ASE heart-type isozyme, 6PF 2 K/Fru 2 6 P2ASE heart type isozyme, 6PF-2-K/Fru-2, F262_HUMAN, Fructose 2 6 bisphosphatase cardiac isozyme, Fructose-2, PFK 2/FBPase 2, PFK/FBPase 2, PFKFB cardiac, PFKFB2.

Background: PFKFB2 is involved in both the synthesis and degradation of fructose-2,6-bisphosphate, a regulatory molecule that controls glycolysis in eukaryotes. It has a 6-phosphofructo-2-kinase activity that catalyzes the synthesis of fructose-2,6-bisphosphate, and a fructose-2,6-biphosphatase activity that catalyzes the degradation of fructose-2,6-bisphosphate. PFKFB2 regulates fructose-2,6-bisphosphate levels in the heart, while a related enzyme encoded by a different gene regulates fructose-2,6-bisphosphate levels in the liver and muscle. This enzyme functions as a homodimer. There are two named isoforms.

Gene ID: 5208

Pathways: PI3K-Akt Signaling, Positive Regulation of Peptide Hormone Secretion, Regulation of Carbohydrate Metabolic Process