MEF2B antibody

Catalog No. ABIN7504178

Product Details anti-MEF2B Antibody

Target: MEF2B (Myocyte Enhancer Factor 2B (MEF2B))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MEF2B antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunoprecipitation (IP)

Specificity: The myocyte enhancer factor-2 (MEF-2) family of transcription factors associate with co-repessors or co-activators to regulate development and function of T cells, neuronal cells, and muscle cells. Four family members, termed MEF-2A, -2B, -2C, and -2D, arise from alternatively spliced transcripts. These members bind as homo- and heterodimers to the MEF-2 site in the promoter region of affected genes. Differential regulation in the expression of the four transcripts implies functional distinction for each during embryogenesis and development. The process of differentiation from mesodermal precursor cells to myoblasts has led to the discovery of a variety of tissue-specific factors that regulate muscle gene expression. The myogenic basic helix-loop-helix proteins, including MyoD, myogenin, Myf-5, and MRF4, are one class of identified factors. The MEF-2 family represents a second class of DNA binding regulatory proteins. Each of these proteins binds to the MEF-2 target DNA sequence present in the regulatory regions of many muscle-specific genes.

Cross-Reactivity (Details): Human.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full-length human MEF2B protein

Isotype: IgG2a

Application Details

Application Notes:

Positive Control: HeLa or Ramos cells.

Known Application: Immunoprecipitation (1-2 μg per 100-500 μg of total protein), Western Blot (1-2 μg/mL),Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), ,Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA and 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody is stable for 24 months. Non-hazardous. Also available WITHOUT BSA & azide at 1.0mg/ml.

Expiry Date: 24 months

Target Details for MEF2B

Target: MEF2B (Myocyte Enhancer Factor 2B (MEF2B))

Alternative Name: MEF2B (MEF2B Products)

Synonyms: si:ch211-125m10.1 antibody, AI451606 antibody, RSRFR2 antibody, myocyte enhancer factor 2b antibody, myocyte enhancer factor 2B antibody, mef2b antibody, MEF2B antibody, Mef2b antibody

Background: MADS box transcription enhancer factor 2, MDS1 EVI1, Mef2b, Myocyte enhancer factor 2B, Myocyte-specific enhancer factor 2B, PRDM3, RSRFR2, Serum response factor like protein 2, XMEF2,MEF2B (Transcriptional Activator)
Cellular localisation: Nucleus.

Molecular Weight: 160kDa

Gene ID: 100271849, 4207, 153629

UniProt: Q02080