Mesothelin antibody (AA 273-407) (Biotin)

Catalog No. ABIN7126105

This anti-Mesothelin antibody is a Mouse Monoclonal antibody detecting Mesothelin in IHC (f). Suitable for Human.

Quick Overview for Mesothelin antibody (AA 273-407) (Biotin) (ABIN7126105)

Target: Mesothelin (MSLN)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Mesothelin antibody is conjugated to Biotin

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Product Details anti-Mesothelin Antibody (Biotin)

Binding Specificity: AA 273-407

Specificity: Mesothelin is a 40 kDa glycosyl-phosphatidylinositol-anchored glycoprotein cleaved from a 69 kDa precursor protein. Mesothelin immunoreactivity is high in cancers of the ovary (serous papillary, endometrioid and undifferentiated) and pancreas, with less frequent staining seen in adenocarcinomas of the endometrium, lung and stomach/esophagus. Mesothelin is one of the most sensitive markers for mesothelioma.

Cross-Reactivity (Details): Human, Mouse and Rat.

Purification: Antibody purified from Bioreactor Concentrate by Protein A/G and conjugated to Biotin.

Immunogen: Recombinant fragment (around aa 273-407) of human Mesothelin (MSLN) protein (exact sequence is proprietary)

Isotype: IgG2b

Application Details

Application Notes:

Positive Control: CAPAN-2 or HeLa cells. Lung Mesothelioma.

Known Application: Immunohistochemistry (Formalin-fixed) (2-4 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 100 μg/mL

Buffer: Antibody purified from Bioreactor Concentrate by Protein A/G and conjugated to Biotin. Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody is stable for 24 months. Non-hazardous. Also available WITHOUT BSA & azide at 1.0mg/ml.

Expiry Date: 24 months

Target Details for Mesothelin

Target: Mesothelin (MSLN)

Alternative Name: MSLN

Background: CAK1, Megakaryocyte potentiating factor, Mesothelin, MSLN, SMR, SMRP,Mesothelin (Mesothelial Marker)
Cellular localisation: Cell Surface and Secreted

Molecular Weight: 40kDa (Mature form), 69kDa (Precursor)

Gene ID: 10232, 408488

UniProt: Q13421

Pathways: EGFR Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process