ECD/SGT1 antibody

Catalog No. ABIN7126219

The Mouse Monoclonal anti-ECD/SGT1 antibody (Clone PCRP-ECD-1D10) (ABIN7126219) specifically detects ECD/SGT1 in IF and FACS. The antibody is reactive with Human samples.

Quick Overview for ECD/SGT1 antibody (ABIN7126219)

Target: ECD/SGT1 (ECD) (Ecdysoneless Homolog (ECD))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ECD/SGT1 antibody is un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: PCRP-ECD-1D10

Product Details anti-ECD/SGT1 Antibody

Purpose: ECD/ SGT1 Antibody

Specificity: Inhibits MDM2-mediated degradation of p53/TP53 possibly by cooperating in part with TXNIP. May be involved transcriptional regulation. In vitro has intrinsic transactivation activity enhanced by EP300. May be a transcriptional activator required for the expression of glycolytic genes. Involved in regulation of cell cycle progression. Proposed to disrupt Rb-E2F binding leading to transcriptional activation of E2F proteins. The cell cycle -regulating function may depend on its RUVBL1-mediated association with the R2TP complex. May play a role in regulation of pre-mRNA splicing.

Cross-Reactivity (Details): Human. Predicted to react with Rabbit, Goat, Horse, Guinea pig, Dog, Pig, Rhesus monkey, Orangutan and Elephant (based on sequence homology).

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full-length human ECD protein

Isotype: IgG2b

Application Details

Application Notes:

Known_Application: Immunoprecipitation (1-2 μg per 100-500 μg of total protein), ,Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), ,Optimal dilution for a specific application should be determined.

Positive_Control: HeLa or 293T cells. Human testis, spleen or colon.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for ECD/SGT1

Target: ECD/SGT1 (ECD) (Ecdysoneless Homolog (ECD))

Alternative Name: ECD

Background:

Ecdysoneless homolog, hSGT1, Human suppressor of GCR two, Protein ecdysoneless homolog, SGT1 protein

HeLa or 293T cells.Human testis, spleen or colon.

Molecular Weight: 72.76kDa

Gene ID: 11319, 631822

UniProt: O95905

Pathways: Regulation of Carbohydrate Metabolic Process