Recombinant Decorin antibody (AA 212-336)

Catalog No. ABIN7126285

The Rabbit Monoclonal anti-Decorin antibody (Clone DCN-7031R) (ABIN7126285) specifically detects Decorin in IHC. The antibody is reactive with Human samples.

Quick Overview for Recombinant Decorin antibody (AA 212-336) (ABIN7126285)

Target: Decorin (DCN)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Decorin antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: DCN-7031R

Product Details anti-Decorin Antibody

Binding Specificity: AA 212-336

Purpose: Recombinant Decorin Antibody

Specificity: Decorin is a small leucine-rich proteoglycan (SLRP) family member that consists of a glycosaminoglycan chain-containing core protein. The core protein contains ten leucine rich repeats that contain sites for glycosylation, flanked by disulfide bond stabilizing loops. Decorin binds to Collagen Type I, II and IV in vivo and promotes the formation of fibers with variations in stability and solubility. The Decorin core protein binds to growth factors, intercellular matrix molecules, such as Fibronectin and Thrombospondin, and to the Decorin endocytosis receptor. Decorin binds to and inhibits TGF æ and is a direct or indirect negative modulator of TGF æ synthesis. Inhibition of Decorin core protein gene expression by the combination of IFN-Á and TNF°™ may contribute to cartilage destruction that is characteristic of inflammatory joint diseases. For immunostaining, pre-incubation with chondroitinase-SBC or testicular hyaluronidase may be required to expose the epitope.

Cross-Reactivity (Details): Human.

Purification: 200ug/ml of Ab produced in CHO cell mammalian-based expression system.

Immunogen: Recombinant human Decorin protein fragment (around aa212-336) (exact sequence is proprietary)

Isotype: IgG, kappa

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Human prostate or skin tissue (IHC).

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Decorin

Target: Decorin (DCN)

Alternative Name: DCN

Background:

Bone proteoglycan II, CSCD, Dermatan sulphate proteoglycans II (DSPG2), PG40, PGII, PGS2, Proteoglycan core protein, SLRR1B, Small leucine rich protein 1B

Human prostate or skin tissue (IHC).

Molecular Weight: 43kDa

Gene ID: 1634, 728830

UniProt: P07585

Pathways: Glycosaminoglycan Metabolic Process