AKR1C1 antibody

Catalog No. ABIN7126291

The Mouse Monoclonal anti-AKR1C1 antibody (ABIN7126291) specifically detects AKR1C1 in WB and IHC (f). The antibody is reactive with Human samples.

Quick Overview for AKR1C1 antibody (ABIN7126291)

Target: AKR1C1 (DDH) (Dihydrodiol Dehydrogenase (DDH))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This AKR1C1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Product Details anti-AKR1C1 Antibody

Specificity: DDH / AKR1C1 is a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the reaction of progesterone to the inactive form 20-alpha-hydroxy-progesterone.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant human full-length AKR1C1 protein

Isotype: IgG1

Application Details

Application Notes:

Known_Application: Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: HepG2 or A549 cells. Human liver or stomach tissue.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for AKR1C1

Target: AKR1C1 (DDH) (Dihydrodiol Dehydrogenase (DDH))

Alternative Name: AKR1C1

Background: 2-dihydrobenzene-1, 2-diol dehydrogenase, 20 alpha (3 alpha) hydroxysteroid dehydrogenase, 20ALPHAHSD, 2ALPHAHSD, AK1C1, C9, Chlordecone reductase homolog HAKRC, DD1/DD2, DDH1, Dihydrodiol dehydrogenase 1/2, H37, HBAB, Hepatic dihydrodiol dehydrogenase, High affinity hepatic bile acid-binding protein, Indanol dehydrogenase, MBAB, Trans-1,2 dihydrobenzene 1,2 diol dehydrogenase, Type II 3 alpha hydroxysteroid dehydrogenase,Aldo-keto Reductase Family 1 Member C1 / DD1
Cellular localisation: Cytoplasm.

Molecular Weight: 37kDa

Gene ID: 1645, 460260

UniProt: Q04828

Pathways: Steroid Hormone Biosynthesis, C21-Steroid Hormone Metabolic Process