E4F1 antibody

Catalog No. ABIN7126308

The Mouse Monoclonal anti-E4F1 antibody has been validated for IF and FACS. It is suitable to detect E4F1 in samples from Human.

Quick Overview for E4F1 antibody (ABIN7126308)

Target: E4F1 (E4F Transcription Factor 1 (E4F1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This E4F1 antibody is un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-E4F1 Antibody

Specificity: E4F1 (E4F transcription factor 1), also known as E4F, is a 784 amino acid protein that localizes to both the nucleus and the cytoplasm and contains 9 C2H2-type zinc fingers. Expressed ubiquitously in adult and fetal tissues, E4F1 exists as a homodimer that binds DNA and is thought to act as a transcriptional repressor and may also play a role in cell survival and growth via cell cycle control. Additionally, E4F1 is thought to function as a ubiquitin ligase that mediates the ubiquitination (and subsequent degradation) of target proteins and may be involved in the p53 tumor suppressor pathway. E4F1, which may be post-translationally phosphorylated or sumoylated, is subject to proteolytic cleavage which results in the creation of a short peptide with specific DNA binding capabilities.

Cross-Reactivity (Details): Human

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human E4F1 protein

Isotype: IgG1

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), ,Immunofluorescence (1-2 μg/mL), ,Optimal dilution for a specific application should be determined.

Positive_Control: HeLa, Jurkat or MCF7 cells.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for E4F1

Target: E4F1 (E4F Transcription Factor 1 (E4F1))

Alternative Name: E4F1

Background: E4F transcription factor 1, isoform CRA_b, P120E4F transcription factor, p50E4F, Putative E3 ubiquitin-protein ligase E4F1, RING type E3 ubiquitin transferase E4F1, Transcription factor E4F1,E4F1
Cellular localisation: Nucleus.

Molecular Weight: 83kDa

Gene ID: 1877, 513268

UniProt: Q66K89