EIF2C3 antibody

Catalog No. ABIN7126312

Product Details anti-EIF2C3 Antibody

Target: EIF2C3 (Eukaryotic Translation Initiation Factor 2C3 (EIF2C3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This EIF2C3 antibody is un-conjugated

Application: ELISA, Immunoprecipitation (IP), Immunofluorescence (IF), Coating (Coat), Flow Cytometry (FACS)

Specificity: This antigen is involved in binding and translation repression of short RNAs. This antigen is also known as argonaute RISC catalytic component 3.Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) and represses the translation of mRNAs which are complementary to them. Proposed to be involved in stabilization of small RNA derivates (siRNA) derived from processed RNA polymerase III-transcribed Alu repeats containing a DR2 retinoic acid response element (RARE) in stem cells and in the subsequent siRNA-dependent degradation of a subset of RNA polymerase II-transcribed coding mRNAs by recruiting a mRNA decapping complex involving EDC4. Possesses RNA slicer activity but only on select RNAs bearing 5'- and 3'-flanking sequences to the region of guide-target complementarity.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human AGO3 protein

Isotype: IgG1

Application Details

Application Notes:

Known_Application: ELISA (For coating, order antibody without BSA), ,Immunoprecipitation (1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)), ,Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), ,Optimal dilution for a specific application should be determined.

Positive_Control: HeLa, MCF7 or U-2OS cells.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for EIF2C3

Target: EIF2C3 (Eukaryotic Translation Initiation Factor 2C3 (EIF2C3))

Alternative Name: AGO3 (EIF2C3 Products)

Synonyms: EIF2C3 antibody, Eif2c3 antibody, Argonaute3 antibody, ago3 antibody, eif2c3 antibody, eif2c3b antibody, si:dkey-3n22.3 antibody, AW048688 antibody, C130014L07Rik antibody, ARGONAUTE 3 antibody, T19E23.8 antibody, T19E23_8 antibody, argonaute 3, RISC catalytic component antibody, argonaute RISC catalytic component 3b antibody, argonaute RISC catalytic subunit 3 antibody, argonaute RISC catalytic component 3 antibody, ARGONAUTE 3 antibody, AGO3 antibody, Ago3 antibody, ago3b antibody

Background: Argonaute 3, Argonaute3, eIF 2C 3, eIF-2C 3, eIF2C 3, Eif2c3, EIF2C3 protein, Eukaryotic translation initiation factor 2C 3, Eukaryotic translation initiation factor 2C3, FLJ12765, hAgo3, MGC86946, Protein argonaute-3,AGO3 (Argonaute-3) / eIF2C3
Cellular localisation: Cytoplasm>P-bodies. Nucleoplasm.

Molecular Weight: 97.36kDa

Gene ID: 192669, 657659

UniProt: Q9H9G7

Pathways: Fc-epsilon Receptor Signaling Pathway, Regulatory RNA Pathways, EGFR Signaling Pathway, Neurotrophin Signaling Pathway