alpha 2 Macroglobulin antibody (AA 604-748)

Catalog No. ABIN7126326

The Mouse Monoclonal anti-alpha 2 Macroglobulin antibody is suitable to detect alpha 2 Macroglobulin in samples from Human. It has been validated for IHC.

Quick Overview for alpha 2 Macroglobulin antibody (AA 604-748) (ABIN7126326)

Target: alpha 2 Macroglobulin (A2M) (alpha-2-Macroglobulin (A2M))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This alpha 2 Macroglobulin antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: A2M-3621

Product Details anti-alpha 2 Macroglobulin Antibody

Binding Specificity: AA 604-748

Purpose: Alpha-2-Macroglobulin Antibody

Specificity: °™-2-Macroglobulin (°™-2M) is a homotetrameric serum protein consisting of four identical subunits that form dimers through disulfide bonds. Initially, °™-2M was characterized as a pan-proteinase inhibitor that was able to bait proteinases into cleaving specific peptide sequences on °™-2M. This interaction induces a conformational change in °™-2M, thus enabling it to trap the proteinase and further inhibit its activity. Subsequently, °™-2M has been shown to function as a carrier protein and regulator of cytokines during inflammation. Circulating transforming growth factor æ (TGF æ) in serum is primarily bound to °™-2M, which renders TGF æ inactive. °™-2M also binds to IL-6 and, thereby, increases the concentration of IL-6 near lymphocytes, hepatocytes and stem cells involved in mediating the inflammatory cascade. Mutations and deletions in the gene encoding °™-2M are associated with an increased incidence of Alzheimer s disease (AD), which is consistent with the role of °™-2M in mediating the clearance and degradation of A æ, the major component of æ-Amyloid deposits accumulated during AD.

Cross-Reactivity (Details): Human.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Human recombinant A2M protein fragment (around aa604-748) (exact sequence is proprietary)

Isotype: IgG2c, kappa

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Human liver or placenta tissues.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for alpha 2 Macroglobulin

Target: alpha 2 Macroglobulin (A2M) (alpha-2-Macroglobulin (A2M))

Alternative Name: A2M

Background:

C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5, Alpha-2-M (A2M), CPAMD5

Human liver or placenta tissues.

Molecular Weight: 185kDa

Gene ID: 2, 212838

UniProt: P01023

Pathways: Lipid Metabolism