ELK1 antibody

Catalog No. ABIN7126329

The Mouse Monoclonal anti-ELK1 antibody has been validated for ELISA and IP. It is suitable to detect ELK1 in samples from Human.

Quick Overview for ELK1 antibody (ABIN7126329)

Target: ELK1 (ELK1, Member of ETS Oncogene Family (ELK1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ELK1 antibody is un-conjugated

Application: ELISA, Immunoprecipitation (IP)

Product Details anti-ELK1 Antibody

Specificity: Ets-1 is the prototype member of a family of genes identified on the basis of homology to the v-Ets oncogene isolated from the E26 erythroblastosis virus. This family of genes currently includes Ets-1, Ets-2, Erg-1 3, Elk-1, Elf-1, Elf-5, NERF, PU.1, PEA3, ERM, FEV, ER8l, Fli-1, TEL, Spi-B, ESE-1, ESE-3A, Net, ABT1and ERF. Members of the Ets gene family exhibit varied patterns of tissue expression, and share a highly conserved carboxy-terminal domain containing a sequence related to the SV40 large T antigen nuclear localization signal sequence. This conserved domain is essential for Ets-1 binding to DNA and is likely to be responsible for the DNA binding activity of all members of the Ets gene family. Several of these proteins have been shown to recognize similar motifs in DNA that share a centrally located 5'-GGAA-3' element.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human ELK1 protein

Isotype: IgG2a

Application Details

Application Notes:

Known_Application: ELISA, Immunoprecipitation (1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)), ,Optimal dilution for a specific application should be determined.

Positive_Control: HeLa or K562 cells.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for ELK1

Target: ELK1 (ELK1, Member of ETS Oncogene Family (ELK1))

Alternative Name: ELK1

Background: ETS transcription factor, ELK2 member of ETS oncogene family, ETS domain-containing protein Elk-1, Member of ETS oncogene family, Tyrosine kinase (ELK1) oncogene,ELK1
Cellular localisation: Nucleus.

Molecular Weight: 44.89kDa

Gene ID: 2002, 181128

UniProt: P19419

Pathways: MAPK Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling