Recombinant ALDH1A1 antibody (AA 315-434)

Catalog No. ABIN7126364

This Rabbit Monoclonal antibody specifically detects ALDH1A1 in WB and IHC. It exhibits reactivity toward Human, Mouse, Rat, Guinea Pig and Hamster.

Quick Overview for Recombinant ALDH1A1 antibody (AA 315-434) (ABIN7126364)

Target: ALDH1A1 (Aldehyde Dehydrogenase 1 Family, Member A1 (ALDH1A1))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse, Rat, Guinea Pig, Hamster

Host: Rabbit

Clonality: Monoclonal

Conjugate: This ALDH1A1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: ALDH1A1-7011R

Product Details anti-ALDH1A1 Antibody

Binding Specificity: AA 315-434

Purpose: Recombinant ALDH1A1 (Aldehyde Dehydrogenase 1A1) Antibody

Specificity: ALDH1A1 belongs to the ALDH enzymes, a family of evolutionarily conserved enzymes comprised of 19 isoforms that are localized in the cytoplasm, mitochondria or nucleus. ALDH1A1 is predominantly expressed in the epithelium of testis, brain, eye, liver, kidney, as well as neural and hematopoietic stem cells. Reportedly, high ALDH1A1 expression is found in solitary fibrous tumor (SFT) and hemangiopericytoma (HPC), compared to meningiomas and synovial sarcomas. In combination with CD34, ALDH1A1 may be useful for the differentiation among SFT, HPC, meningioma, and synovial sarcoma.

Cross-Reactivity (Details): Human.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment (around aa 315-434) of human ALDH1A1 protein (exact sequence is proprietary).

Isotype: IgG, kappa

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: K562, HepG2 or A549 cells. Human colon or testicular carcinoma.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for ALDH1A1

Target: ALDH1A1 (Aldehyde Dehydrogenase 1 Family, Member A1 (ALDH1A1))

Alternative Name: ALDH1A1

Background:

AHD2, ALDC, Aldehyde dehydrogenase 1 soluble, ALDH-E1, epididymis luminal protein 12, epididymis luminal protein 9, epididymis secretory sperm binding protein Li53e, HEL-S-53e, PUMB1, RALDH1, Retinal dehydrogenase 1

HepG2 or A549 cells. Human colon, testicular carcinoma., K562 or Human Liver, Mouse Liver, Rat Liver, Hamster Liver or Guinea pig Liver tissue lysates

Molecular Weight: 56kDa

Gene ID: 216, 76392

UniProt: P00352

Pathways: Dopaminergic Neurogenesis