FABP1 antibody (AA 1-127)

Catalog No. ABIN7126371

The Mouse Monoclonal anti-FABP1 antibody has been validated for WB, IHC and IHC (f). It is suitable to detect FABP1 in samples from Human.

Quick Overview for FABP1 antibody (AA 1-127) (ABIN7126371)

Target: FABP1 (Fatty Acid Binding Protein 1, Liver (FABP1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This FABP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Product Details anti-FABP1 Antibody

Binding Specificity: AA 1-127

Specificity: Fatty acid-binding proteins, designated FABPs, are a family of homologous cytoplasmic proteins that are expressed in a highly tissue-specific manner and play an integral role in the balance between lipid and carbohydrate metabolism. FABPs mediate fatty acid (FA) and/or hydrophobic ligand uptake, transport and targeting within their respective tissues. The mechanisms underlying these actions can give rise to both passive diffusional uptake and protein-mediated transmembrane transport of FAs. FABPs are expressed in adipocytes (A-FABP), brain (B-FABP), epithelium (E-FABP, psoriasis-associated FABP, PA-FABP), striated muscle and heart (H-FABP, mammary-derived growth inhibitor or MDGI), intestine (I-FABP), liver (L-FABP or FABP1), myelin (M-FABP) and testis (T-FABP). FABP1 (L-FABP) expression is modulated by developmental, hormonal, dietary and pharmacological factors, and is required for cholesterol synthesis and metabolism.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Human recombinant FABP1 protein fragment (around aa1-127) (exact sequence is proprietary)

Isotype: IgG2b

Application Details

Application Notes:

Known_Application: Western Blot (2-4 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Liver or colon carcinoma tissues (IHC). Human kidney tissue lysate (WB).

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for FABP1

Target: FABP1 (Fatty Acid Binding Protein 1, Liver (FABP1))

Alternative Name: FABP1

Background: FABP1, L-FABP,Fatty Acid Binding Protein (Liver) / FABP1
Cellular localisation: Cytoplasm. Nucleus.

Molecular Weight: 14kDa

Gene ID: 2168, 380135

UniProt: P07148

Pathways: Chromatin Binding, Regulation of Lipid Metabolism by PPARalpha