Fibronectin 1 antibody

Catalog No. ABIN7126423

This anti-Fibronectin 1 antibody is a Mouse Monoclonal antibody detecting Fibronectin 1 in WB, IF, IHC (f) and IP. Suitable for Human.

Quick Overview for Fibronectin 1 antibody (ABIN7126423)

Target: Fibronectin 1 (FN1)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Fibronectin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunoprecipitation (IP)

Product Details anti-Fibronectin 1 Antibody

Specificity: Fibronectin is an extracellular matrix glycoprotein present on most cell surfaces, in extracellular fluids and in plasma. A high molecular weight heterodimeric protein, it was originally discovered as a protein missing from the surfaces of virus-transformed cells, and it has been shown to be involved in various functions including cell adhesion, cell motility and wound healing. Alternative splicing and glycosylation give rise to several different forms of Fibronectin, some of which exhibit restricted tissue distribution or association with malignancies. It has been shown that myofibroblast phenotype formation correlates with the occurrence of glycosylated Fibronectin and Fibronectin splice variants in Dupuytren's disease.

Cross-Reactivity (Details): Human. Monkey. Porcine. Bovine. Equine. Avian. Amphibian.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Human plasma fibronectin

Isotype: IgG1

Application Details

Application Notes:

Known_Application: Immunoprecipitation (1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)), ,Western Blot (1-2 μg/mL),Immunofluorescence (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: SW156 cells. Human kidney.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for Fibronectin 1

Target: Fibronectin 1 (FN1)

Alternative Name: FN1

Background: Cold insoluble globulin (CIG), FINC, FN1, FNZ, GFND, GFND2, LETS, Migration stimulating factor (MSF), Ugl-Y3,Fibronectin (Total)
Cellular localisation: Connective tissue matrix

Molecular Weight: 220kDa (monomer), 440kDa (dimer)

Gene ID: 2335, 203717

UniProt: P02751

Pathways: Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Autophagy