Fibronectin 1 antibody

Catalog No. ABIN7126426

This anti-Fibronectin 1 antibody is a Mouse Monoclonal antibody detecting Fibronectin 1 in IF, FACS and IHC (f). Suitable for Human.

Quick Overview for Fibronectin 1 antibody (ABIN7126426)

Target: Fibronectin 1 (FN1)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Fibronectin 1 antibody is un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Product Details anti-Fibronectin 1 Antibody

Specificity: Fibronectins are disulfide-linked, dimeric glycoproteins of ~440 kDa. They possess at least four binding sites for collagen, glycosaminoglycans, transglutaminase, and a cell surface receptor. Epitope of this MAb is located in the 29 kDa N-terminal fibrin- and heparin-binding domain of fibronectin. It does not cross-react with MMP-2, MMP-9, or TIMP-2. Fibronectins are involved in cell adhesion, tissue organization, and wound healing. Fibronectins are present in basement membranes, interstitial connective tissue matrix, and blood. Cellular fibronectin is widely distributed in the stroma of many malignant tumors.

Cross-Reactivity (Details): Human and Pig.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: High molecular weight proteins secreted by cultivated human fibroblasts

Isotype: IgG2a

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Positive_Control: SW156 cells. Prostate or Kidney.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for Fibronectin 1

Target: Fibronectin 1 (FN1)

Alternative Name: FN1

Background: Cold insoluble globulin (CIG), FINC, FN1, FNZ, GFND, GFND2, LETS, Migration stimulating factor (MSF), Ugl-Y3,Fibronectin (Fibrin/Heparin-Binding Domain)
Cellular localisation: Connective tissue matrix

Molecular Weight: 220kDa (monomer), 440kDa (dimer)

Gene ID: 2335, 203717

UniProt: P02751

Pathways: Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Autophagy