SIRT3 antibody

Catalog No. ABIN7126428

The Mouse Monoclonal anti-SIRT3 antibody has been validated for WB, ELISA, IP, IF, FACS and Coat. It is suitable to detect SIRT3 in samples from Human.

Quick Overview for SIRT3 antibody (ABIN7126428)

Target: SIRT3 (Sirtuin 3 (SIRT3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SIRT3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF), Flow Cytometry (FACS), Coating (Coat)

Product Details anti-SIRT3 Antibody

Specificity: Zinc-fingers and homeobox (ZHX) proteins are transcription factors that interact with the activation domain of the A subunit of nuclear factor-Y (NF-YA). ZHX1-3 are ubiquitously expressed proteins expressed in various tissues. They act as transcriptional repressors and localize to the nucleus. The ZHX proteins contain two Cys2-His2-type zinc-finger motifs and five homeodomains (HDs). These domains allow the ZHX proteins to form homodimers, but they can also form heterodimers with each other. However, this dimerization is not required for repressor activity. Hypermethylation-mediated silencing of ZHX2 is an epigenetic event involved in hepatocellular carcinoma (HCC).

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human SIRT3 protein

Isotype: IgG2a

Application Details

Application Notes:

Known_Application: ELISA (For coating, order antibody without BSA), ,Immunoprecipitation (1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)), ,Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), ,Optimal dilution for a specific application should be determined.

Positive_Control: HeLa cells.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for SIRT3

Target: SIRT3 (Sirtuin 3 (SIRT3))

Alternative Name: SIRT3

Background: HSIRT3, Mitochondrial nicotinamide adenine dinucleotide dependent deacetylase, NAD dependent deacetylase sirtuin 3 mitochondrial, Regulatory protein SIR2 homolog 3, SIR2 like protein 3, SIR2-like protein 3, SIR2L3, Sirtuin silent mating type information regulation 2 homolog 3 (S. cerevisiae), Sirtuin type 3, Sirtuin3,Sirtuin 3 (SIRT3)
Cellular localisation: Mitochondrion matrix.

Molecular Weight: 43.57kDa

Gene ID: 23410, 716456

UniProt: Q9NTG7