Recombinant AMH antibody (AA 460-560)

Catalog No. ABIN7126472

This Rabbit Monoclonal antibody specifically detects AMH in IHC (f). It exhibits reactivity toward Human.

Quick Overview for Recombinant AMH antibody (AA 460-560) (ABIN7126472)

Target: AMH (Anti-Mullerian Hormone (AMH))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This AMH antibody is un-conjugated

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Product Details anti-AMH Antibody

Binding Specificity: AA 460-560

Specificity: The transforming growth factor i² (TGFi²) superfamily is composed of numerous growth and differentiation factors, including TGFi²1-3, Mullerian inhibiting substance (MIS), growth/differentiation factor (GDF) 1-9, bone morphogenic protein (BMP) 2-8, glial cell line-derived neurotrophic factor (GDNF), Inhibin i±,i²-A, i²-B and i²-C, Lefty and Nodal. Members of the TGFi² superfamily are involved in embryonic development and adult tissue homeostasis. The MIS glycoprotein is produced by the Sertoli cells of the testes. Fetal testes produce both MIS and testosterone, the presence of which result in male offspring. Absence of MIS and testosterone in a developing fetus results in the induction of Mullerian duct differentiation, and Wolffian duct development is not induced. Testosterone induces the differentiation of the Wolffian ducts whereas MIS causes regression of the Muellerian duct. MIS inhibits the growth of tumors derived from tissues of Mullerian duct origin. MIS can also inhibit the autophosphorylation of the EGF receptor in vitro. Defects in anti-Muellerian hormone are the cause of persistent Muellerian duct syndrome type I (PMDS-1). PMDS-1 is a form of male pseudo hermaphroditism characterized by a failure of Muellerian duct regression in otherwise normal males.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: A synthetic peptide from the C-terminus of human AMH (aa 460-560) (exact sequence is proprietary)

Isotype: IgG

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Human ovary or testis.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for AMH

Target: AMH (Anti-Mullerian Hormone (AMH))

Alternative Name: AMH

Background: Muellerian inhibiting factor (MIF),Anti Mullerian Hormone (AMH) / Mullerian Inhibiting Substance (MIS)
Cellular localisation: Secreted

Molecular Weight: 70kDa (reduced), 140kDa (nonreduced)

Gene ID: 268, 112432

Pathways: Negative Regulation of Hormone Secretion