GCLM antibody (Regulatory Subunit)

Catalog No. ABIN7126478

This Mouse Monoclonal antibody specifically detects GCLM in WB and IHC. It exhibits reactivity toward Human.

Quick Overview for GCLM antibody (Regulatory Subunit) (ABIN7126478)

Target: GCLM (Glutamate-Cysteine Ligase, Modifier Subunit (GCLM))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This GCLM antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: CPTC-GCLM-1

Product Details anti-GCLM Antibody

Binding Specificity: Regulatory Subunit

Purpose: Glutamate-Cysteine Ligase Regulatory Subunit Antibody

Specificity: GCLM known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia.

Cross-Reactivity (Details): Human.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant human full-length GCLM protein

Isotype: IgG2a, kappa

Application Details

Application Notes:

Known_Application: Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: A549, HepG2 or HeLa cell lysates. Human muscle and liver tissue.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for GCLM

Target: GCLM (Glutamate-Cysteine Ligase, Modifier Subunit (GCLM))

Alternative Name: GCLM

Background:

GCLM Antibody, Gamma-ECS regulatory subunit Antibody, GLCLR Antibody, GCS light chain Antibody, GSC light chain Antibody

A549, HepG2 or HeLa cell lysates. Human muscle and liver tissue.

Molecular Weight: 31kDa

Gene ID: 2730, 315562

UniProt: P48507