Recombinant Interferon gamma antibody

Catalog No. ABIN7126540

The Rabbit Monoclonal anti-Interferon gamma antibody (Clone IFNG-3996R) (ABIN7126540) specifically detects Interferon gamma in IHC. The antibody is reactive with Human samples.

Quick Overview for Recombinant Interferon gamma antibody (ABIN7126540)

Target: Interferon gamma (IFNG)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Interferon gamma antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: IFNG-3996R

Product Details anti-Interferon gamma Antibody

Purpose: Recombinant Interferon gamma (IFNG) Antibody

Specificity: Recognizes a protein of 20-25 kDa, identified as human interferon. This MAb is specific to human IFN-Á and recognizes both recombinant and native human IFN-gamma. T lymphocytes and NK cells mainly produce IFN-Á. It is a pleiotropic cytokine involved in the regulation of nearly all phases of immune and inflammatory responses, including the activation, growth and differentiation of T cell, B cells, macrophages, NK cells and other cell types such as endothelial cells and fibroblasts. It has weak anti-viral and anti-proliferative activity, and potentiates the antiviral and anti-tumor effects of IFN-°™/ æ (type I interferon).

Cross-Reactivity (Details): Human.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full-length human IFNG protein

Isotype: IgG, kappa

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: PMA stimulated human peripheral blood mononuclear cells. Renal or testicular carcinoma.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Interferon gamma

Target: Interferon gamma (IFNG)

Alternative Name: IFNG

Background:

AMCF1, Beta thromboglobulin like protein, CXC chemokine ligand 8 (CXCL8), Emoctakin, Granulocyte chemotactic protein 1 (GCP1), IFG, IFI, IFN Immune, Interleukin 8, LECT, LUCT, Lymphocyte derived neutrophil activating factor, LYNAP, Macrophage Activating Factor (MAF), Monocyte derived neutrophil activating protein (MONAP), Monocyte derived neutrophil chemotactic factor (MDNCF), Neutrophil activating factor (NAF), Neutrophil activating peptide 1 (NAP1), Neutrophil activating protein 1 (NAP1), SCYB8, T cell chemotactic factor, T Cell Interferon

PMA stimulated human peripheral blood mononuclear cells. Renal or testicular carcinoma.

Molecular Weight: 20-25kDa

Gene ID: 3458, 856

UniProt: P01579

Pathways: Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy