IL1A antibody (AA 113-271)

Catalog No. ABIN7126559

This Mouse Monoclonal antibody specifically detects IL1A in IHC (f). It exhibits reactivity toward Human.

Quick Overview for IL1A antibody (AA 113-271) (ABIN7126559)

Target: IL1A (Interleukin 1 alpha (IL1A))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IL1A antibody is un-conjugated

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Product Details anti-IL1A Antibody

Binding Specificity: AA 113-271

Specificity: Two forms of interleukin-1, designated IL-1i±and IL-1i², have been described. Although encoded by distinct genes and exhibiting roughly only 25 % sequence identity, IL-1i± and IL-1i² bind to the same receptor and seem to elicit similar biological responses. IL-1 production is generally thought to be associated with inflammation, but it has also been shown to be expressed during kidney development, thymocyte differentiation and cartilage degradation. IL-1 plays a critical role in the regulation of immune response and inflammation, acting as an activator of T and B lymphocytes and natural killer (NK) cells. In T cells, IL-1 stimulates the production of IL-2 and selectively inhibits IL-4 expression. IL-1 induces B cell proliferation and maturation, and immunoglobulin synthesis. NK cells require IL-1i² for production of the anti-pathogen IFN-i³. IL-1 has also been implicated in several pathological conditions including rheumatoid arthritis, inflammatory bowel disease and atherosclerosis.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment (around aa 113-271) of human IL1A protein

Isotype: IgG2b

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: HeLa cells. Human colon carcinoma tissue.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for IL1A

Target: IL1A (Interleukin 1 alpha (IL1A))

Alternative Name: IL1A

Background: BAF, FAF, Hematopoietin 1, IL 1 alpha, IL1F1, Interleukin 1 alpha, LAF, LEM, Preinterleukin 1 alpha, Pro interleukin 1 alpha,Interleukin-1 alpha (IL-1 alpha)
Cellular localisation: Secreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretor

Molecular Weight: 33kDa (proprotein), 17kDa (mature)

Gene ID: 3552, 1722

UniProt: P01583

Pathways: NF-kappaB Signaling, Autophagy, Cancer Immune Checkpoints