IL-3 antibody

Catalog No. ABIN7126574

This Mouse Monoclonal antibody specifically detects IL-3 in IHC (f). It exhibits reactivity toward Human.

Quick Overview for IL-3 antibody (ABIN7126574)

Target: IL-3 (Interleukin 3 (IL-3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IL-3 antibody is un-conjugated

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Product Details anti-IL-3 Antibody

Specificity: Interleukin-3, or IL-3, is a pleiotropic cytokine that is primarily secreted by activated T lymphocytes and stimulates the proliferation and differentiation of hematopoietic cells. IL-3 not only supports growth of both pluripotent stem cells and the more differentiated committed progenitors, but it also stimulates the functional activity of some fully differentiated cells. IL-3 has also been shown to protect mast cells from undergoing apoptosis. IL-3 exerts its biological effects through a receptor which consists of a ligand-specific i± subunit and a signal transducing i² subunit common to the IL-3/IL-5/GM-CSF receptors. The carboxy terminus of the i² subunit has been shown to be necessary for activation of the MAP kinase signaling pathway. Although the IL-3 receptor has no intrinsic kinase activity, stimulation with IL-3 leads to tyrosine phosphorylation of the JAK/Tyk 2 family member, JAK2, which in turn activates and causes nuclear translocation of Stat5a and Stat5b.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment of human IL3 protein (exact sequence is proprietary)

Isotype: IgG2b

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Human thymus or tonsil tissue.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for IL-3

Target: IL-3 (Interleukin 3 (IL-3))

Alternative Name: IL3

Background: Colony stimulating factor multiple, Hematopoietic growth factor, Interleukin 3 (colony stimulating factor, multiple), Mast cell growth factor, MCGF, Multi CSF, Multilineage colony stimulating factor, P cell stimulating factor,Interleukin-3 (IL-3)
Cellular localisation: Secreted.

Molecular Weight: 17kDa

Gene ID: 3562, 694

Pathways: JAK-STAT Signaling, Regulation of Carbohydrate Metabolic Process, Autophagy