PDX1 antibody

Catalog No. ABIN7126596

The Mouse Monoclonal anti-PDX1 antibody has been validated for IF and FACS. It is suitable to detect PDX1 in samples from Human.

Quick Overview for PDX1 antibody (ABIN7126596)

Target: PDX1 (Pancreatic and Duodenal Homeobox 1 (PDX1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PDX1 antibody is un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-PDX1 Antibody

Specificity: Pancreatic duodenal homeobox-1 protein (PDX-1), also designated Insulin promoter factor (IPF1), Insulin upstream factor 1 (IUF1), somatostatin transactivating factor-1 (STF-1) and glucose-sensitive factor (GSF), is a 282 amino acid homeodomain-containing transcription factor present in pancreatic i²-cells. PDX-1 is a key regulator of pancreatic islet development and Insulin gene transcription in i²-cells. PDX-1 is expressed in all cells at the early stages of development and is mainly restricted to the pancreas and duodenum in adult. HNF-3i², HNF-1i± and SP1 positively regulate the PDX-1 enhancer element. PDX-1 is also regulated by Glucagon-like peptide through activation of adenylyl cyclase, which results in an increase in intracellular cAMP activity. The increased levels of cAMP, and the resulting activation of PKA, lead to an increase in PDX-1 transcription and translocation of the protein to the nuclei of i²-cells. PDX-1 binds to the sequence C(C/T) and can heterodimerize with PBX. PDX-1 is phosphorylated by the SAPK2 pathway under high glucose concentrations. Mutations in the PDX-1 gene can cause maturity-onset diabetes of the young and pancreatic agenesis. The gene which encodes PDX-1 maps to human chromosome 13q12.2.

Cross-Reactivity (Details): Human

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human PDX1 protein

Isotype: IgG2

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), ,Immunofluorescence (1-2 μg/mL), ,Optimal dilution for a specific application should be determined.

Positive_Control: HeLa or HEK293 cells.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for PDX1

Target: PDX1 (Pancreatic and Duodenal Homeobox 1 (PDX1))

Alternative Name: PDX1

Background: Glucose sensitive factor, GSF, IDX1, Insulin promoter factor 1 homeodomain transcription factor, IPF1, STF1, IUF1, Islet/duodenum homeobox 1, MODY4, Pancreas/duodenum homeobox, Somatostatin transactivating factor 1,Pancreatic duodenal homeobox-1 protein (PDX1)
Cellular localisation: Nucleus.

Molecular Weight: 46kDa

Gene ID: 3651, 32938

UniProt: P52945

Pathways: Nuclear Receptor Transcription Pathway, Positive Regulation of Peptide Hormone Secretion, Steroid Hormone Mediated Signaling Pathway, Hormone Transport, Carbohydrate Homeostasis, Chromatin Binding, Maintenance of Protein Location