Recombinant Liver Arginase antibody (AA 300-400)

Catalog No. ABIN7126610

This Rabbit Monoclonal antibody specifically detects Liver Arginase in WB and IHC (f). It exhibits reactivity toward Human.

Quick Overview for Recombinant Liver Arginase antibody (AA 300-400) (ABIN7126610)

Target: Liver Arginase (ARG1) (Arginase, Liver (ARG1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Liver Arginase antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Product Details anti-Liver Arginase Antibody

Binding Specificity: AA 300-400

Specificity: Recognizes a protein of 35-38 kDa, which is identified as Arginase 1 (ARG1). Arginase is a manganese metallo-enzyme that catalyzes the hydrolysis of arginine to generate ornithine and urea. Arginase I and II are isoenzymes which differ in subcellular localization, regulation, and possibly function. Arginase I is a cytosolic enzyme, which is expressed mainly in the liver as part of the urea cycle, whereas arginase II is a mitochondrial protein found in a variety of tissues. Antibody to ARG-1 labels hepatocytes in normal tissues and granulocytes in peripheral blood. ARG-1 is a sensitive and specific marker for identification of hepatocellular carcinoma.

Cross-Reactivity (Details): Human. Others not known.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant human ARG1 protein fragment (aa300-400) (Exact sequence is proprietary)

Isotype: IgG

Application Details

Application Notes:

Known_Application: Western Blotting (2-4 μg/mL),Immunohistology (Formalin-fixed) (2-4 μg/mL for 30 minutes at RT) ,(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: 293T cells. Hepatocellular Carcinoma (HCC).

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for Liver Arginase

Target: Liver Arginase (ARG1) (Arginase, Liver (ARG1))

Alternative Name: ARG1

Background: Arginase 1, ARG1, liver-type arginase, type I arginase,Arginase 1 (Hepatocellular Carcinoma Marker)
Cellular localisation: Cytoplasmic

Molecular Weight: 35-38kDa

Gene ID: 383, 440934

UniProt: P05089

Pathways: Cellular Response to Molecule of Bacterial Origin