Recombinant KRT6A antibody

Catalog No. ABIN7126617

The Rabbit Monoclonal anti-KRT6A antibody has been validated for IHC (f). It is suitable to detect KRT6A in samples from Human.

Quick Overview for Recombinant KRT6A antibody (ABIN7126617)

Target: KRT6A (Keratin 6A (KRT6A))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This KRT6A antibody is un-conjugated

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Product Details anti-KRT6A Antibody

Specificity: Cytokeratin 6 is an epidermis-specific type I keratin involved in wound healing. Involved in the activation of follicular keratinocytes after wounding, while it does not play a major role in keratinocyte proliferation or migration. This protein always paired with the type-I keratin K16.Cytokeratin 6 have been identified in epidermis only in samples from plantar glabrous skin while in hairy skin this keratin appeared to be absent in interfollicular epidermis but were clearly present in hair follicle outer root sheath, and this protein also consistently expressed in non-keratinizing stratified squamous epithelia. Among tumors, cytokeratin 6 is typically and strongly expressed in squamous cell carcinomas of different sites, preferentially in inner, maturing layers of the tumor cell nests.Cytokeratin 6 is always used with cytokeratin 5 and is a useful aid for classification of epithelioid mesotheliomas.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment of human KRT6 (exact sequence is proprietary)

Isotype: IgG

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: HeLa cells. Human tonsil or basal cell carcinoma (BCC).

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for KRT6A

Target: KRT6A (Keratin 6A (KRT6A))

Alternative Name: KRT6A

Background: CK6A, CK6B, CK6C, CK6D, CK6E, Keratin Type II Cytoskeletal 6A, Keratin Type II Cytoskeletal 6B, Keratin Type II,Cytokeratin 6A (KRT6A) (Basal Cell Marker)
Cellular localisation: Cytoplasm

Molecular Weight: 56kDa

Gene ID: 3853, 700779

UniProt: P02538