SMAD9 antibody

Catalog No. ABIN7126668

The Mouse Monoclonal anti-SMAD9 antibody is suitable to detect SMAD9 in samples from Human. It has been validated for IF and FACS.

Quick Overview for SMAD9 antibody (ABIN7126668)

Target: SMAD9 (SMAD Family Member 9 (SMAD9))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SMAD9 antibody is un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: PCRP-SMAD9-2F4

Product Details anti-SMAD9 Antibody

Purpose: SMAD9 Antibody

Specificity: Smad proteins, the mammalian homologs of the Drosophila mothers against dpp (Mad) have been implicated as downstream effectors of TGF æ/BMP signaling. Smad1 (also designated Madr1 or JV4-1), Smad5 and mammalian Smad8 (also designated Smad9 or MadH6) are effectors of BMP2 and BMP4 function while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGF æ and activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/TGF æ signaling by interfering with TGF æ-mediated phosphorylation of other Smad family members.

Cross-Reactivity (Details): Human.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full-length human SMAD9 protein

Isotype: IgG1, kappa

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), ,Immunofluorescence (1-2 μg/mL), ,Optimal dilution for a specific application should be determined.

Positive_Control: HeLa, U87 or Ramos cells.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for SMAD9

Target: SMAD9 (SMAD Family Member 9 (SMAD9))

Alternative Name: SMAD9

Background:

MAD homolog 9, Madh6, Mothers against decapentaplegic homolog 9, Mothers against DPP homolog 9, SMAD family member 9, SMAD, mothers against DPP homolog 9 (Drosophila), SMAD8A, SMAD8B, Smad9

HeLa, U87 or Ramos cells.

Molecular Weight: 52.4kDa

Gene ID: 4093, 123119

UniProt: O15198