MBP antibody (AA 119-131)

Catalog No. ABIN7126670

This Mouse Monoclonal antibody specifically detects MBP in WB, IHC, ELISA and IF. It exhibits reactivity toward Human, Rat, Mouse, Guinea Pig, Cow, Hamster and Rabbit.

Quick Overview for MBP antibody (AA 119-131) (ABIN7126670)

Target: MBP

Reactivity: Human, Rat, Mouse, Guinea Pig, Cow, Hamster, Rabbit

Host: Mouse

Clonality: Monoclonal

Conjugate: This MBP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Clone: MBP-4272

Product Details anti-MBP Antibody

Binding Specificity: AA 119-131

Purpose: Myelin Basic Protein Antibody

Specificity: Myelin basic protein (MBP) is the second most abundant protein in central nervous system (CNS) myelin: it comprises 30 % of the total protein and about 10 % of the dry weight of myelin. It is the only structural protein found so far to be essential for formation of CNS myelin, and has been called the executive molecule of myelin . MBP can interact with a number of polyanionic proteins including actin, tubulin, calmodulin, and clathrin, and negatively charged lipids, and acquires structure on binding to them. It may act as a membrane actin-binding protein, which might allow it to participate in transmission of extracellular signals to the cytoskeleton in oligodendrocytes and tight junctions in myelin. MBP may be applicable as a marker for oligodendrogliomas. MBP/4272 recognizes an epitope in the 119-131 region of MBP, useful in clinical diagnosis to detect MBP levels in human, rat and cow MBP.

Cross-Reactivity (Details): Human, bovine and rat, reacts weakly with rabbit. Does not react with guinea pig.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recognizes bovine BMP, in the region of residues 119-131 (GAEGQRPGFGYGG).

Isotype: IgG1

Application Details

Application Notes:

Known_Application: ELISA (For coating, order antibody without BSA), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: U87 cells. Human brain or astrocytoma tissue.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for MBP

Target: MBP

Background:

GDB, Golli MBP, myelin basic protein, Hemopoietic MBP, HMBPR, HUGO, MLD, Myelin A1 Protein, basic, Myelin Deficient, Myelin membrane encephalitogenic protein, SHI, Shiverer, SP

U87 cells. Human brain or astrocytoma tissue. Mouse Brain, Rat Brain, Guinea pig Brain or Hamster Brain

Molecular Weight: 14-22kDa

Gene ID: 4155, 551713

UniProt: P02686