MCM2 antibody (AA 650-750)

Catalog No. ABIN7126677

Product Details anti-MCM2 Antibody

Target: MCM2 (Minichromosome Maintenance Complex Component 2 (MCM2))

Binding Specificity: AA 650-750

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MCM2 antibody is un-conjugated

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Specificity: The specificity of this monoclonal antibody to its intended target was validated by HuProtTM Array, containing more than 19,000, full-length human proteins. MCM7 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that is essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumor suppressor protein RB1/RB.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment (aa 650-750) of human MCM2 protein (exact sequence is proprietary)

Isotype: IgG1

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: MCF-7, Jurkat or Ramos cells. Human tonsil.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for MCM2

Target: MCM2 (Minichromosome Maintenance Complex Component 2 (MCM2))

Alternative Name: MCM2 (MCM2 Products)

Background: BM28, CCNL1, CDC like 1, cdc19, CDCL1, Cell devision cycle like 1, Cyclin like 1, DNA replication licensing factor MCM2, KIAA0030, Minichromosome maintenance complex component 2, Minichromosome maintenance deficient 2 (mitotin), Minichromosome maintenance protein 2 homolog, Mitotin, Nuclear protein BM28,MCM2 (ProliferationMarker)
Cellular localisation: Nuclear

Molecular Weight: 102kDa

Gene ID: 4171, 477481

UniProt: P49736

Pathways: DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA