Recombinant CD99 antibody

Catalog No. ABIN7126689

The Mouse Monoclonal anti-CD99 antibody has been validated for IHC (f). It is suitable to detect CD99 in samples from Human.

Quick Overview for Recombinant CD99 antibody (ABIN7126689)

Target: CD99

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD99 antibody is un-conjugated

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Product Details anti-CD99 Antibody

Specificity: Recognizes a sialoglycoprotein of 27-32 kDa, identified as CD99, or MIC2 gene product, or E2 antigen. MIC2 gene is located in the pseudo-autosomal region of the human X and Y chromosome. MIC2 gene encodes two distinct proteins, which are produced by alternative splicing of the CD99 gene transcript and are identified as bands of 30 and 32 kDa (p30/32).Although its function is not fully understood, CD99 is implicated in various cellular processes including homotypic aggregation of T cells, upregulation of T cell receptor and MHS molecules, apoptosis of immature thymocytes and leukocyte diapedesis.CD99 is expressed on the cell membrane of some lymphocytes, cortical thymocytes, and granulosa cells of the ovary. Most pancreatic islet cells, Sertoli cells of the testis, and some endothelial cells express this antigen. Mature granulocytes express very little or no CD99. MIC2 is strongly expressed on Ewing s sarcoma cells and primitive peripheral neuroectodermal tumors.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human MIC2 protein

Isotype: IgM

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: MOLT-4 cells. Pancreas or Ewing s sarcoma

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for CD99

Target: CD99

Alternative Name: CD99

Background: 12E7, E2 antigen, MIC 2X, MIC 2Y, MIC2, Protein MIC2, Surface antigen MIC2, T-cell surface glycoprotein E2,CD99 / MIC2 (Ewing s Sarcoma Marker)
Cellular localisation: Cell Surface

Molecular Weight: 27-32kDa

Gene ID: 4267, 653349

UniProt: P14209