Recombinant Geminin antibody (AA 71-202)

Catalog No. ABIN7126767

The Rabbit Monoclonal anti-Geminin antibody has been validated for IHC (f). It is suitable to detect Geminin in samples from Human.

Quick Overview for Recombinant Geminin antibody (AA 71-202) (ABIN7126767)

Target: Geminin (GMNN) (Geminin, DNA Replication Inhibitor (GMNN))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Geminin antibody is un-conjugated

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Product Details anti-Geminin Antibody

Binding Specificity: AA 71-202

Specificity: Geminin is a nuclear protein that regulates the initiation of DNA replication during the cell cycle. DNA replication requires the coordinated association of Cdc6 and minichromosome maintenance (MCM) proteins with chromatin. Geminin blocks this assembly of the MCM into the prereplication complex and, in turn, prevents replication from occurring. Expression of Geminin fluctuates throughout the cell cycle with Geminin levels lowest at G1. Throughout S, G2 and M phases, Geminin levels are consistently elevated followed by a decrease during mitosis. The initiation of DNA replication is dependent on the degradation of Geminin during mitosis and the absence of Geminin throughout G1 phase. Geminin degradation is mediated by the anaphase-promoting complex (APC), which specifically targets B-type cyclins and other proteins containing a destruction box motif for degradation by ubiquitin-mediated proteolysis. While geminin expression is essential in maintaining chromosomal integrity, it is frequently overexpressed in cancers and evidence suggests that it plays a significant role in tumor proliferation and progression.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment (around aa71-202) of human GMNN protein (exact sequence is proprietary)

Isotype: IgG1

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: HEK293, HeLa or MCF-7 cells. Human tonsil, testis or triple-negative breast cancer tissue.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for Geminin

Target: Geminin (GMNN) (Geminin, DNA Replication Inhibitor (GMNN))

Alternative Name: GMNN

Background: DNA replication inhibitor, Gem, Geminin DNA replication inhibitor, GMNN, RP3 369A17.3,Geminin / DNA Replication Inhibitor
Cellular localisation: Nuclear expression in highly proliferating cells.

Molecular Weight: 35kDa

Gene ID: 51053, 234896

UniProt: O75496

Pathways: EGFR Signaling Pathway, DNA Replication