PIK3R2 antibody (AA 520-720)

Catalog No. ABIN7126793

This Mouse Monoclonal antibody specifically detects PIK3R2 in IHC (f). It exhibits reactivity toward Human.

Quick Overview for PIK3R2 antibody (AA 520-720) (ABIN7126793)

Target: PIK3R2 (PI3K p85b) (Phosphoinositide 3 Kinase, p85 beta (PI3K p85b))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PIK3R2 antibody is un-conjugated

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Product Details anti-PIK3R2 Antibody

Binding Specificity: AA 520-720

Specificity: Phosphatidylinositol 3-kinase (PI 3-kinase) is composed of p85 and p110 subunits. p85 lacks PI 3-kinase activity and acts as an adapter, coupling p110 to activated protein tyrosine kinase. Two forms of p85 have been described (p85i± and p85i²), each possessing one SH3 and two SH2 domains. Various p110 isoforms have been identified. p110i± and p110i² interact with p85i±, and p110i± has also been shown to interact with p85i² in vitro. p110 L expression is restricted to white blood cells. It has been shown to bind p85i± and p85i², but it apparently does not phosphorylate these subunits. p110 L seems to have the capacity to autophosphorylate. p110i³ does not interact with the p85 subunits. It has been shown to be activated by i± and i²i³ heterotrimeric G proteins.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment of human PIK3R2 protein (around aa 520-720) (exact sequence is proprietary)

Isotype: IgG2b

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Human spleen or bladder.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for PIK3R2

Target: PIK3R2 (PI3K p85b) (Phosphoinositide 3 Kinase, p85 beta (PI3K p85b))

Alternative Name: PIK3R2

Background: P85 beta, P85B, Phosphatidylinositol 3 kinase, regulatory subunit, polypeptide 2 (p85 beta), Phosphatidylinositol 3-kinase 85 kDa regulatory subunit beta, Phosphoinositide 3 kinase regulatory subunit polypeptide 2 (p85 beta), PI3 kinase p85 beta subunit, PI3K regulatory subunit beta, PIK3R2, PtdIns-3-kinase regulatory subunit p85-beta,PIK3R2 / Phosphoinositide-3-kinase regulatory subunit 2
Cellular localisation: Cytoplasm > golgi apparatus. Nucleus.

Molecular Weight: 85kDa

Gene ID: 5296, 371344

UniProt: O00459

Pathways: VEGF Signaling, BCR Signaling